2010

T. VERNE LEE, J. SHAUN LOTT, RICHARD D. JOHNSON & VICKERY L. ARCUS.


Expression and purification of an adenylation domain from a eukaryotic nonribosomal peptide synthetase: Using structural genomics tools for a challenging target.


Protein Expression and Purification 74, 162-168 (IF=1.56)


http://dx.doi.org/10.1016/j.prep.2010.08.004



DOMINIK WIEMUTH, J. SHAUN LOTT, KEVIN LY, YING KE, PAUL TEESDALE-SPITTLE, PETER SNYDER & FIONA J. MCDONALD.


Interaction of Serum- and Glucocorticoid regulated Kinase 1 (SGK1) with the WW domains of Nedd4-2 is required for epithelial sodium channel regulation.”


PLoS ONE, 5, e12163 (IF=4.35)


http://dx.plos.org/10.1371/journal.pone.0012163.



ALEXANDRA MANOS-TURVEY, ESTHER M. M. BULLOCH, PETER J. RUTLEDGE, EDWARD N. BAKER, J. SHAUN LOTT & RICHARD J. PAYNE.Inhibition Studies of Mycobacterium tuberculosis Salicylate Synthase (MbtI).”


ChemMedChem, 5, 1067–1079 (IF=3.23)


http://dx.doi.org/10.1002/cmdc.201000137



SHARON L KENDALL, PHILIPPA BURGESS, RICARDO BALHANA, MIKE WITHERS, ANNEMIEKE TEN BOKUM, J. SHAUN LOTT, CHEN GAO, IRIA UHIA CASTRO, & NEIL G. STOKER. “Cholesterol utilisation in mycobacteria is controlled by two TetR-type transcriptional regulators; kstR and kstR2.”


Microbiology, 156, 1362-1371 (IF=2.84)


http://dx.doi.org/10.1099/mic.0.034538-0



T. VERNE LEE, LINDA J. JOHNSON, RICHARD D. JOHNSON, ALBERT KOULMAN, GEOFFREY A. LANE, J. SHAUN LOTT & VICKERY L. ARCUS


“The structure of a eukaryotic non-ribosomal peptide synthetase adenylation domain which activates a large hydroxamate amino acid in siderophore biosynthesis.”


Journal of Biological Chemistry, 285, 2415-2427 (IF=5.81)


http://dx.doi.org/10.1074/jbc.M109.071324

2009

Nicole J. Moreland, Caroline Charlier, Andrew Dingley, Edward N. Baker & J. Shaun Lott

Making sense of a missense mutation: Characterization of MutT2, a Nudix hydrolase from Mycobacterium tuberculosis, and the G58R mutant encoded in W Beijing strains of M. tuberculosis.

Biochemistry,
48, 699-708 (IF=3.37)

http://pubs.acs.org/doi/abs/10.1021/bi8009554

2008

Miriam L. Sharpe, Chen Gao, Sharon L. Kendall, Edward N. Baker & J. Shaun Lott

The structure and unusual protein chemistry of Hypoxic Response Protein 1, a latency antigen and highly-expressed member of the DosR regulon in M. tuberculosis

Journal of Molecular Biology, 383, 822–836 (IF=4.47)

http://dx.doi.org/10.1016/j.jmb.2008.07.001

2007

Rachael M. Goldstone, Nicole J. Moreland, Ghader Bashiri, Edward N. Baker & J. Shaun Lott

"A new Gateway® vector and expression protocol for fast and efficient recombinant protein expression in Mycobacterium smegmatis."

Protein Expression and Purification, 57, 81-87
(IF=1.69)

http://dx.doi.org/10.1016/j.pep.2007.08.015


SHARON L. KENDALL, MIKE WITHERS, CATHERINE N. SOFFAIR, NICOLE J. MORELAND, SUDAGAR GURCHA, BEN SIDDERS, ROSANGELA FRITA, ANNEMIEKE TEN BOKUM, GURDYAL S. BESRA, J. SHAUN LOTT & NEIL G. STOKER.

“A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis

Molecular Microbiology, 65, 684-699 (IF=5.63)

http://dx.doi.org/10.1111/j.1365-2958.2007.05827.x


A.C. MURILLO, T.C. ALBER, E.N. BAKER, J.M. BERGER, L.T. CHERNEY, M.M. CHERNEY, D. EISENBERG, C. R. GAREN, L. W. HUNG, T.R. IOERGER, W.R. JACOBS, M.N.G. JAMES, C. KIM, I. KRIGER, H. LI, J.S. LOTT, R. SANKARANARAYANAN, B.W. SEGELKE, T.C. TERWILLIGER, F. WANG, J.C. SACCHETTINI.

High Throughput Crystallography of TB Drug Targets.”

Infectious Disorders - Drug Targets, 7, 127-139 (IF=4.27)

2006

ANTHONY J. HARRISON, MINMIN YU, THERÉS GÅRDENBORG, MARTIN MIDDLEDITCH, ROCHELLE J. RAMSAY, EDWARD N. BAKER & J. SHAUN LOTT

"The Structure of MbtI from Mycobacterium tuberculosis, the First Enzyme in the Biosynthesis of the Siderophore Mycobactin, Reveals it to be a Salicylate Synthase "

Journal of Bacteriology 188, 6081-6091 (IF=3.99)


J. SHAUN LOTT, BLAKE PAGET, JODIE M. JOHNSTON, LOUIS T. J. DELBAERE, JILL A. SIMON-SIGRELL, MARK J. BANFIELD, AND EDWARD N. BAKER

"The structure of an ancient conserved domain establishes a structural basis for stable histidine phosphorylation and identifies a new family of adenosine specific kinases."

Journal of Biological Chemistry, 281, 22131–22141 (IF=5.81)


VICKERY L. ARCUS, J. SHAUN LOTT, JODIE M. JOHNSTON & EDWARD N. BAKER

"The potential impact of structural genomics on Mycobacterium tuberculosis drug discovery."

Drug Discovery Today, 11, 28-34 (IF=7.15)


CLARE LEE, CATHERINE GOODFELLOW, FARAH JAVID-MAJD, EDWARD N. BAKER & J. SHAUN LOTT

"The crystal structure of TrpD, a metabolic enzyme essential for lung colonization by Mycobacterium tuberculosis, in complex with its substrate phosphoribosylpyrophosphate."

Journal of Molecular Biology, 355, 784-797 (IF=4.89)

2005

CELIA J. WEBBY, HEATHER M. BAKER, J. SHAUN LOTT, EDWARD N. BAKER & EMILY J. PARKER.

"The structure of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase from Mycobacterium tuberculosis reveals a common catalytic scaffold and ancestry for Type I and Type II enzymes."

Journal of Molecular Biology, 354, 927–939 (IF=4.89)


NICOLE MORELAND, RACHAEL ASHTON, HEATHER M. BAKER, IVAN IVANOVIC, SEAN PATTERSON, VICKERY L. ARCUS, EDWARD N. BAKER & J. SHAUN LOTT

"A Flexible and Economical Medium-Throughput Strategy for Protein Production and Crystallization."

Acta Crystallographica Section D, 61, 1378-1385 (IF=1.69)


MIRIAM L. SHARPE, EDWARD N. BAKER & J. SHAUN LOTT

"Crystallization of Hypoxic Response Protein I (HRPI) from Mycobacterium tuberculosis Using Dehydration."

Acta Crystallographica Section F: Structural Biology and Crystallization Communications 61, 565-568


CELIA J. WEBBY, J. SHAUN LOTT, HEATHER M. BAKER, EDWARD N. BAKER & EMILY J. PARKER

"Crystallization and preliminary X-ray crystallographic analysis of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Mycobacterium tuberculosis."

Acta Crystallographica Section F: Structural Biology and Crystallization Communications 61, 403-406


ANTHONY J. HARRISON, ROCHELLE J. RAMSAY, EDWARD N. BAKER &
J. SHAUN LOTT

"Crystallization and preliminary X-ray crystallographic analysis of MbtI, a protein essential for siderophore biosynthesis in Mycobacterium tuberculosis."

Acta Crystallographica Section F: Structural Biology and Crystallization Communications 61, 121-123


SIMON LI, NEIL A. PETERSON, MIN-YOUNG KIM, CHANG-YUB KIM, LI-WEI HUNG, MINMIN YU, TIMOTHY LEKIN, BRENT W. SEGELKE, J. SHAUN LOTT* & EDWARD N. BAKER*

"Crystal Structure of AhpE from Mycobacterium tuberculosis, a 1-Cys peroxiredoxin."

Journal of Molecular Biology 346, 1035–1046 (IF=4.89)

*Corresponding authors

Older

2004

J. SHAUN LOTT & EDWARD N. BAKER

Using structural genomics to understand Mycobacterium tuberculosis”

IUBMB Life, 56, 113–117 (IF=2.32)


2003

J. Shaun Lott, Mark J. Banfield, Jill A. Sigrell, Edward N.Baker.

“Making the most of two crystals: structural analysis of a conserved hypothetical protein using native gel screening and SAD phasing.”

Acta Crystallographica Section D 59, 2242–2246 (IF=1.69)


EDWARD N. BAKER, VICKERY L. ARCUS AND J. SHAUN LOTT

“Protein structure prediction and analysis as a tool for functional genomics”

Applied Bioinformatics, 2, S3-S10


T.C. TERWILLIGER, M.S. PARK, G.S. WALDO, J. BERENDZEN, L.-W. HUNG, C.-Y. KIM, C.V. SMITH, J.C. SACCHETTINI, M. BELLINZONI, R. BOSSI, E. DE ROSSI, A. MATTEVI, A. MILANO, G. RICCARDI, M. RIZZI, M.M. ROBERTS, A.R. COKER, G. FOSSATI, P. MASCAGNI, A.R.M. COATES, S.P. WOOD, C.W. GOULDING, M.I. APOSTOL, D.H. ANDERSON, H.S. GILL, D.S. EISENBERG, B. TANEJA, S. MANDE, E. POHL, V. LAMZIN, P. TUCKER, M. WILMANNS, C. COLOVOS, W. MEYER-KLAUCKE, A.W. MUNRO, K.J. MCLEAN, K.R. MARSHALL, D. LEYS, J.K. YANG, H.-J. YOON, B.I. LEE, M.G. LEE, J.E. KWAK, B.W. HAN, J.Y. LEE, S.-H. BAEK, S.W. SUH, M.M. KOMEN, V.L. ARCUS, E.N. BAKER, J.S. LOTT, W. JACOBS JR., T. ALBER, B. RUPP

“The TB structural genomics consortium: a resource for Mycobacterium tuberculosis biology”

Tuberculosis 83, 223–249 (IF=3.43)


LOTT J. S. & BAKER E. N.

“Functional genomics of Mycobacterium tuberculosis: New insights into mankind’s deadliest pathogen”

New Zealand Science Review 60, 43-47


2002

GOULDING, C. W., APOSTO, M., ANDERSON, D. H., GILL, H. S., SMITH, C. V., KUO, M. R., YANG, J. K., WALDO G. S., SUH, S., CHAUHAN, R., KALE, A., BACHHAWAT, N., MANDE, S. C., JOHNSTON, J. M., LOTT, J. S., BAKER, E. N., ARCUS, V. L., LEYS, D., MCLEAN, K. J., MUNRO, A. W., BERENDZEN, J., SHARMA, V., PARK, M. C., EISENBERG, D., SACCHETTINI, J., ALBER, T., RUPP, B., JACOBS, W. AND TERWILLIGER, T. C.

“The TB Structural Genomics Consortium: Providing a Structural Foundation for Drug Discovery”

Current Drug Targets - Infectious Disorders, 2, 121-141 (IF=4.27)


LOTT J. S., CODDINGTON-LAWSON S. J., TEESDALE-SPITTLE P. H. & McDONALD F. J.

“A single WW domain is the predominant mediator of the interaction between the human ubiquitin-protein ligase Nedd4 and the human epithelial sodium channel.”

Biochemical Journal 361, 481-488.


LOO T., PATCHETT M. L., NORRIS G. E. & LOTT J. S.

“Using Secretion to Solve a Solubility Problem: High-yield Expression in E. coli and Purification of the Bacterial Glycoamidase PNGase F.”

Protein Expression and Purification, 24, 90-98. (IF=1.87)


2001

BANFIELD, M. J., LOTT, J. S., ARCUS, V. L., McCARTHY, A. A., & BAKER, E. N.

“Structure of HisF, a histidine biosynthetic protein from Pyrobaculum aerophilum

Acta Crystallographica Section D 57, 1518-1525. (IF=1.69)


2000

LOTT J. S., HALBIG D., BAKER H. M., HARDMAN M. J., SPRENGER G. A. & BAKER E. N.

“Crystal structure of a truncated mutant of glucose-fructose oxidoreductase shows that an N‑terminal arm controls tetramer formation.”

Journal of Molecular Biology. 304, 575-584. (IF=4.89)


1999

LOTT J. S., WILDE J. I., CARNE A., EVANS N. & FINDLAY J. B. C.

“The Ordered Visual Transduction Complex of the Squid Photoreceptor Membrane”

Molecular Neurobiology 20, 1-20. (IF=3.76)


1997

ATTWOOD, T. K. , AVISON, H. , BECK, M. E.., BEWLEY, M. , BLEASBY, A. J. , BREWSTER, F. , COOPER, P. , DEGTYARENKO, K. , GEDDES, A. J. , FLOWER, D. R. , KELLY, M. P. , LOTT, J. S., MEASURES, K. M. , PARRY-SMITH, D. J. , PERKINS, D. N. , SCORDIS, P. , SCOTT, D. & WORLEDGE, C.

“The PRINTS database of protein fingerprints: a novel information resource for computational molecular biology.”

Journal of Chemical Information & Computer Sciences 37, 417-24.


1996

BEWLEY, M. C., LOTT, J. S., BAKER, E. N. & PATCHETT, M. L.

“The cloning, expression and crystallisation of a thermostable arginase.”

FEBS Letters 386, 215-218. (IF=3.37)


1992

LOTT, J. S., RYBA, N. J. P. , POTTINGER, J. D. , KEEN, J. N. , CARNE, A. & FINDLAY, J. B. C.

“The g-subunit of the principal G-protein from squid (Loligo forbesi) photoreceptors contains a novel N-terminal sequence.”

FEBS Letters 312, 241-4. (IF=3.37)